An empirical selected for intraperitoneal injection methylcellulose (MC) in order to prevent postoperative adhesions of the abdominal cavity to be examined.
Due to the fact that examine the pharmacokinetics and pharmacodynamics of MC is not possible (lack of qualitative reactions) to investigate the response of the internal organs in connection with possible toxic effects on organs of the target. However, given these firms Genzym, which experts using proven methods radionukleidnyh complete elimination of carboxymethylcellulose with intraperitoneal its introduction, we assume that the MC and also completely eliminated from the body.
The purpose of the study: the exclusion of the toxic properties of water-soluble gel MTs during its intraperitoneal administration.
Methyl cellulose is a water-soluble cellulose ether and methyl alcohol. Gel on the basis of its psevdoplastichen, according to the literature has no toxicity at the outer and perroralnom application has biological stability, is physiologically inert.
In the experiment, we used white rats - Wistar males weighing 100 - 120 g. We studied five experimental series of 10 rats and the second series - control - 5 rats.
In sterile conditions operbloka chair of operative surgery and topographic anatomy CSMU rats under ether anesthesia by puncture into the abdominal cavity was introduced to 3 ml 3% sterile gel IC test group and 3 ml of isotonic NaCl solution control group.
Animals of experimental group were derived from the experiment at 1, 3, 7, 14 and 30th day, and the control group - 3 day.
Upon expiration or termination of the experiment was evaluated: the general brand viagra condition of the animal, general and biochemical blood analysis; mortem examination, removal of the corpse of the liver, spleen, cecum, kidney and breast organocomplex their histology by conventional methods with the color slice hematoxylin + eosin and Van- Gieson, light microscopy and photography of the objects being studied. The obtained data were processed statistically. In all series of general condition, complete blood count (erythrocyte sedimentation rate, white blood cell count and indices of leukocyte formula) are statistically not significantly changed. Biochemical blood tests (carried out using standard sets): studied alanine aminotransferase, alkaline phosphatase, creatine kinase, bilirubin and prothrombin index. In the dynamics of blood parameters between control and experience, statistically significant differences and mean values between control and experimental groups, we have not been identified. At autopsy, none of the cases, pathological changes of the abdominal organs were found. Histological examination of all the membrane of the large intestine pronounced structural features have not. Crypts and lamina propria are not violated, the latest massively infiltrated with lymphocytes. Covering epithelium forms a continuous layer of mesothelial lining of flat epithelial cells presented an elongated shape. Infiltration, edema, degeneration and other pathological changes were observed.
The structure of the lung tissue was normal, the lumen of the alveoli is free, capillary-blooded. The structure of the liver lobules is normal, parenchyma is represented radially oriented trabeculae, between which are seen moderate expansion sinusoidal blood capillary. The cytoplasm of the hepatocyte acidophilic, granular structure. Kernels are rounded, with distinct nucleoli. Endothelial cells and macrophages of the liver was normal. Kidney cells of round shape, regular structure, the cavity of the capsule is free. Well defined outer layer capsule of kidney cells. Proximal and distal convoluted tubules are clearly distinguishable, are formed by renal cubic epithelium with rounded nuclei. Between the tubules thin layer of connective tissue. Renal membrane without apparent pathology. The structure of the normal myocardium. Strands of cardiomyocytes are beams, separated by thin prosloykmi connective tissue. Blood vessels are moderately full-bodied. Cardiomyocyte nucleus oval, structured. Mioplazma slaboatsidofilna. Determined by longitudinal myofibrillar striation. Red and white pulp of the spleen has normal structure. Moderate hyperemia. Red pulp is rich in macrophages with inclusions in the form of hemosiderin and other particles.
Thus, when injected into the abdominal cavity Gel MC changes of clinical, biochemical indices of blood and morphological structure of internal organs we have not been identified. The data obtained suggest that the introduction into the abdominal cavity of the offered funds do not cause any pathological changes.
The use of prophylactic drugs on the basis of MC seems quite promising in the fight against post-operative adhesions and their complications and require further experimental study.
stable remission
careful monitoring
complications
inhibitory effects
many years
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